In recent years, agricultural robots have moved away from being pure sensor-carrying platforms for gathering data in the field into becoming action-delivering platforms providing physical interaction with the environment. In this chapter, the actuation methods that are most commonly used on intelligent agricultural robots in order to transform this optimal action into implement behaviour, motion, physical interaction or manipulation are discussed. These actuation methods consist of both traditional actuators that have been transformed into precision farming tools and novel actuators enabled by robotics and autonomous systems. A short introduction to each actuator is presented followed by example uses found in the agricultural domain. The chapter concludes with a case study that looks at how different actuators are used to automate strawberry production.