This chapter reviews the current status of research on feed enzymes with an emphasis on identifying the key challenges researchers face in terms of current trends in enzyme development, mechanism(s) of action and enzyme efficacy. It also addresses new research directions and the development of consensus protocol and/or research approaches to prevent potential duplication of effort and to aid coordinated research and collaboration. Sections cover the key drawbacks and opportunities in the application of phytase, non-starch polysaccharides (NSP) and NSP enzymes, β-mannanase, supplemental α-amylase and protease, with a focus on achieving effective use of feed ingredients and more sustainable production of poultry meat.