Held in Florence in 1929, the First National Exhibition of History of Science was a pivotal event in the shaping of Italian cultural panorama. With more than 8000 items on display coming from public and private lenders, it showed the general public how rich the Italian scientific heritage was and how it could be regarded as part of a general nation-claiming narrative, thus laying the foundation for today’s protection policy and scholarly research. Moreover, it is also a telling case-study that offers precious insights into the complex relationships between cultural enterprises and political power during the fascist era, helping us understand how today’s geography of Italian cultural institutions have been shaped and reshaped through time.